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memerald sec61β  (Addgene inc)


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    Structured Review

    Addgene inc memerald sec61β
    Memerald Sec61β, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+memerald+sec61+c+18/mEmerald-Sec61-C-18+(Plasmid+%2354249)/bio_rxiv__64898__2026__03__23__710523-181-9-10
    Average 94 stars, based on 16 article reviews
    memerald sec61β - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging
    Article Snippet: Some cultures were grown with media supplemented with sodium pyruvate (Gibco, Cat# 11360070). .. For labeling the ER membrane, we expressed the plasmid mEmerald-Sec61-C-18, a gift from Michael Davidson (deceased, formerly Florida State University, Tallahassee, FL; Addgene plasmid # 54249; which encodes GFP-Sec61β). .. For labeling mitochondria, we expressed GFP-OMP25 from a plasmid that was made by modifying pEGFP-C1 (Takara Bio Inc.) to have eGFP fused to the C-terminus of human OMP25/SYNJ2BP cDNA containing the amino acids “QVQNGPIGHRGEGDPSGIPIFMVLVPVFALTMVAAWAFMRYRQQL” and localizes to mitochondria, as shown previously .

    Membrane:

    Article Title: Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging
    Article Snippet: Some cultures were grown with media supplemented with sodium pyruvate (Gibco, Cat# 11360070). .. For labeling the ER membrane, we expressed the plasmid mEmerald-Sec61-C-18, a gift from Michael Davidson (deceased, formerly Florida State University, Tallahassee, FL; Addgene plasmid # 54249; which encodes GFP-Sec61β). .. For labeling mitochondria, we expressed GFP-OMP25 from a plasmid that was made by modifying pEGFP-C1 (Takara Bio Inc.) to have eGFP fused to the C-terminus of human OMP25/SYNJ2BP cDNA containing the amino acids “QVQNGPIGHRGEGDPSGIPIFMVLVPVFALTMVAAWAFMRYRQQL” and localizes to mitochondria, as shown previously .

    Plasmid Preparation:

    Article Title: Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging
    Article Snippet: Some cultures were grown with media supplemented with sodium pyruvate (Gibco, Cat# 11360070). .. For labeling the ER membrane, we expressed the plasmid mEmerald-Sec61-C-18, a gift from Michael Davidson (deceased, formerly Florida State University, Tallahassee, FL; Addgene plasmid # 54249; which encodes GFP-Sec61β). .. For labeling mitochondria, we expressed GFP-OMP25 from a plasmid that was made by modifying pEGFP-C1 (Takara Bio Inc.) to have eGFP fused to the C-terminus of human OMP25/SYNJ2BP cDNA containing the amino acids “QVQNGPIGHRGEGDPSGIPIFMVLVPVFALTMVAAWAFMRYRQQL” and localizes to mitochondria, as shown previously .



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